Methylcholanthrene-induced Sarcoma of the Transplantation Type from Plasma Membranes of a P40-solubilized and Partially Purified Tumor-specific Antigens Biological and Biochemical Properties of Nonidet
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چکیده
SUMMARY Tumor-specific transplantation antigen (TSTA) was solu-bilized from cell membranes of sarcoma Meth-A with non-ionic detergent Nonidet P40. Soluble TSTA was partially characterized by Chromatographie separation and electro-phoresis. The antigen responsible for tumor rejection activ ity had a molecular weight of approximately 70,000 daltons in the presence of detergent and an electrophoretic mobility of a-globulin. TSTA was well separated from mouse histo-compatibility antigen H-2 by lectin affinity chromatography. The antigen purified by a sequence of procedures, includ ing gel filtration, lectin affinity chromatography, column electrophoresis, and rechromatography on agarose, showed only three major bands on polyacrylamide gel elec trophoresis. TSTA was specific for sarcoma Meth-A.
منابع مشابه
Biological and biochemical properties of Nonidet P40-solubilized and partially purified tumor-specific antigens of the transplantation type from plasma membranes of a methylcholanthrene-induced sarcoma.
Tumor-specific transplantation antigen (TSTA) was solubilized from cell membranes of sarcoma Meth-A with non-ionic detergent Nonidet P40. Soluble TSTA was partially characterized by chromatographic separation and electrophoresis. The antigen responsible for tumor rejection activity had a molecular weight of approximately 70,000 daltons in the presence of detergent and an electrophoretic mobilit...
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We reported previously the partial purification of detergent-solubilized specific tumor rejection antigen of a chemically induced sarcoma, Meth-A. During the course of the study, rabbit antiserum against a partially purified specific tumor rejection antigen preparation was raised and rendered specific by in vivo absorption. In this report we show that an antigenic molecule defined by in vivo-ab...
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A tumor-associated transplantation antigen with an apparent molecular weight of 75,000 has been isolated from the cytosol of the BALB/c methylcholanthrene-induced sarcoma, Meth A. The antigen was purified either by preparative electrophoresis in the presence of NaDodSO4 or by immunoaffinity chromatography after hexylamine agarose chromatography, gel filtration, and hydroxylapatite chromatograph...
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تاریخ انتشار 2006